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| Catalog Number | Size | Price (USD) | Shopping Cart |
|---|---|---|---|
| GP10106 | 50 ul | $225.00 | Buy Now | Add to Cart |
| GP10106 | 100 ul | $375.00 | Buy Now | Add to Cart |
| P10104 | 100 ug Blocking Peptide | $95.00 | Buy Now | Add to Cart |
Three types of opioid receptors have been cloned -- mu, delta, and kappa. Opioid receptors are seven transmembrane G-protein coupled receptors. They share a high degree of homology and are most divergent at the N- and C-termini. Activation of mu opioid receptors leads to a decrease in neuronal excitability.
Image: Mu Opioid Receptor staining of Rat Dorsal Horn. Courtesy of Dr. Louis Gendron, University of Sherbrooke.
Protocol:
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Rats were deeply anesthetized with isoflurane and perfused through the aortic arch with 100 ml of heparin (75 U/ml of heparin in 0.9% saline) followed by 100 ml of a mixture of 3.75% acrolein and 2% PFA in 0.1 M PB, pH 7.4, and then by 300 ml of 2% PFA in the same buffer at 45 ml/min.m Lumbar spinal cord was removed and postfixed in 2% PFA in 0.1 M PB for 1 h at 4°C. Sections (50 µm thick) were cut using a Vibratome and processed for MOR labeling.
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Sections were incubated in 1% sodium borohydride for 30 min and extensively rinsed in 0.1 M PB. They were then cryoprotected for 30 min in a solution consisting of 25% sucrose and 3% glycerol in 0.05 M PB and snap frozen with isopentane (–50°C) followed by liquid nitrogen.
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After being rapidly thawed in 0.1 M PB, sections were rinsed with TBS 0.1M and preincubated for 1 h at room temperature in 3% NGS diluted in TBS. They were then incubated for 36 h at 4°C in MOR antiserum diluted 1/500 in TBS containing 0.5% NGS. Sections were then rinsed twice with TBS and incubated for 1 h at room temperature with biotinylated anti-guinea pig antibody (1/400; Vector Laboratories).
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Following three 10 min washes in TBS, sections were incubated 30 min with Vectastain Elite ABC (Vector Laboratories). Sections were rinsed three times with TBS and peroxidase complex revealed for 8 minutes with DAB substrate (2.2 mg/10 ml + 0.01% H2O2).
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At the end of this incubation, sections were washed twice with TBS, mounted on microscope slides, and dehydrated with ethanol.
More Links
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Mu Opioid Receptor Catalog#: RA10104 |
Neurotransmissiom Research Antibodies i-Fect Transfection Kit
gene silencing of DOR, NaV1.8 tetrodotoxin-resistant sodium channel, NTS2 and more in-vitro and in vivo
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WB Images: Representative Western blots of isolated mouse brain striatum and cerebellum for the non-phospho-MOR antibody or the MOR-P2 antibody. The samples from cerebellum demonstrate a lack of IR for both the non-phospho-MOR antibody and MOR-P2 antibody further showing the selectivity of these antibodies. The images are each representative of four independent experiments. Dilution 1:1000. Neuroscience 146 (2007) 1795–1807.