Magic Red™ Real Time! KitsMeasure apoptosis in whole living, intact cells - no lysis required |
Magic Red™ (MR) kits measure apoptosis via active caspases and cathepsins in whole living, intact cells - no lysis required. As apoptosis progresses and caspase activity increases, the red fluorescent signal increases. These unique kits are not ELISAs and do not use antibodies - instead they are based on cell-permeant substrate reagents specifically targeted by active apoptosis enzymes.
Just culture your cells, add MR to the media, and incubate. If the enzymes are active, they will cleave the reagent causing it to fluoresce red. MR kits are true substrate assays - the red fluorescent label is quenched until it is actually cleaved by active enzymes. Only cells with active enzymes will fluoresce, so you don’t get any signal from pro-enzymes or inactive forms of the enzyme.
Image: Caspases 3&7 kit clearly distinguishes apoptotic from non-apoptotic cells. The top 3 THP-1 cells have active caspases (they fluoresce red) the bottom 3 cells do not (they do not fluoresce).
| Name | Catalog # | Type | Species | Applications | Size | Price |
|---|---|---|---|---|---|---|
| Magic Red™ Caspases 3&7 | KF17306 | Kit | 25 Tests | $155 | ||
| Magic Red™ Caspases 3&7 | KF17307 | Kit | 100 Tests | $389 | ||
| Magic Red™ Cathepsin B | KF17308 | Kit | 25 Tests | $155 | ||
| Magic Red™ Cathepsin B | KF17309 | Kit | 100 Tests | $389 | ||
| Magic Red™ Cathepsin K | KF17310 | Kit | 25 Tests | $155 | ||
| Magic Red™ Cathepsin L | KF17312 | Kit | 25 Tests | $155 | ||
| Magic Red™ Cathepsin L | KF17313 | Kit | 100 Tests | $389 |