5HT Serotonins |
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5HT (Serotonin)-Catalog#:RA20000
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Neurotransmission Research Antibodies
Primary Neurons and Astrocytes -Primary human, rat and mouse neurons and astrocytes
Ana C Magalhaes,Kevin D Holmes,Lianne B Dale,Laetitia Comps-Agrar,Dennis Lee,Prem N Yadav,Linsay Drysdale,Michael O Poulter,Bryan L Roth,Jean-Philippe Pin,Hymie Anisman& Stephen S G Ferguson.CRF receptor 1 regulates anxiety behavior via sensitization of 5-HT2 receptor signaling. Nature Neuroscience. doi:10.1038/nn.2529. Published online11 April 2010.
...Brains were harvested, placed in 4% paraformaldehyde in 1× PBS at 4 °C for 4 h, moved to 30% sucrose (wt/vol) in 1× PBS until they sank, brains were then frozen on dry ice and stored at −80 °C. Brain sections (30 μm) were prepared and free-floating sections in 1× PBS (one per well in a 24-well plate) were permeabilized with 0.4% Triton X-100 in 1× PBS for 1 h. 1× PBS /0.4% Triton X-100 containing 0.1% glycine (wt/vol), 0.1% lysine (wt/vol), 1% BSA (wt/vol) and 1% normal donkey serum (wt/vol). Primary antibodies (rabbit polyclonal antibody to 5-HT2A, Neuromics cat # RA24288; goat polyclonal antibody to CRFR1, Abcam cat # ab59023) were incubated in blocking buffer for 72 h at 4 °C. Sections were then washed five times in 1× PBS/0.4% Triton X-100 (10 min each). Hoechst (2–5 μg ml−1) and secondary antibodies (donkey Alexa Fluor 555 conjugated anti-goat (1:500) and donkey Alexa Fluor 488 conjugated anti-rabbit antibodies (1:500); Invitrogen) were diluted in blocking buffer and slices were incubated for 1 h at 20–23 °C. Sections were washed five times in 1× PBS/0.4% Triton X-100 (10 min each). Sections were mounted on slides and visualized by Zeiss LSM-510 META multophoton laser-scanning microscope with a Zeiss 25× NA 1.2 oil immersion lens and appropriate filters...
Cullen L. Schmid, Kirsten M. Raehal, and Laura M. Bohn. Agonist-directed signaling of the serotonin 2A receptor depends on β-arrestin-2 interactions in vivo. Published online on January 14, 2008, 10.1073/pnas.0708862105
... Neurons were incubated with 5-HT2AR polyclonal rabbit (1:100; Neuromics)...
Deborah Conte, Susan A Deuchars, Ferenc Erdelyi, Gabor Szabo, Jim Deuchars. Serotonin immunoreactive terminals closely appose GABAergic neurones in autonomic regions of the spinal cord. University College London 2006 (2006) Proc Physiol Soc 3 PC77
...Thoracic spinal cord was sectioned at 50µm on a vibratome (Leica). Serotoninergic terminals were localised using rabbit anti-5-HT (1:500, Neuromics Inc, USA) and visualised with donkey anti-rabbit Alexa555 (1:1000, Invitrogen, UK)...
Małgorzata Lehner, Ewa Taracha, Anna Skórzewska, Piotr Maciejak, Aleksandra Wisłowska-Stanek, Małgorzata Zienowicz, Janusz Szyndler, Andrzej Bidziński and Adam Płaźnik. Behavioral, immunocytochemical and biochemical studies in rats differing in their sensitivity to pain. doi:10.1016/j.bbr.2006.03.044
...slide-mounted brain sections were incubated in rabbit polyclonal serotonin antiserum diluted at 1:100 (Neuromics) in temperature 4–8 °C for 24 ...
Naohisa Tsujino, Yasushi Nakatani, Yoshinari Seki, Akane Nakasato, Michiko Nakamura, Michiya Sugawara and Hideho Arita. Abnormality of circadian rhythm accompanied by an increase in frontal cortex serotonin in animal model of autism. doi:10.1016/j.neures.2006.10.018
Rong-Jian Liu, Evelyn K. Lambe and George K. Aghajanian. Somatodendritic autoreceptor regulation of serotonergic neurons: dependence on l-tryptophan and tryptophan hydroxylase-activating kinases. European Journal of Neuroscience, Vol. 21, pp. 945–958, 2005
Syann Lee, Christine L. Walker,Barbara Karten, Sharee L. Kuny, Alysa A. Tennese, Megan A. O'Neill and Rachel Wevrick. Essential role for the Prader–Willi syndrome protein necdin in axonal outgrowth. Human Molecular Genetics 2005 14(5):627-637