MOR-1C |
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Pain and Inflammation Research Antibodies
Neurotransmission-Neurotransmission Research Antibody Categories
Primary Neurons and Astrocytes-Primary human, rat and mouse neurons and astrocytes
...Cells were resuspended in Triton X-100 extraction buffer [10 mM Tris, pH 7.5, 120 mM NaCl, 25 mM KCl, 1 mM PMSF, 1 protease inhibitor pellet/10 ml (Roche), 1 mg/ml iodoacetamide, and 0.5% Triton X-100] and solubilized on ice for 2 h. Lysates were cleared by 12,000 rpm spin and then added to avidin-conjugated agarose beads (Pierce) for immunoprecipitation overnight at 4°C with rotation. Immunoprecipitates were centrifuged at 12,000 rpm and washed three times with Triton X-100 extraction buffer and once with PBS plus 0.1% SDS. Immunoprecipitates were washed, and proteins were eluted and resolved as described in the coimmunoprecipitation experiments. Immunoblots were performed using the mOR C-terminal directed antibody (Neuromics, Edina, MN), donkey anti-rabbit secondary antibody, and enhanced chemiluminescence detection...
D. Blivis, G. Z. Mentis, M. J. O'Donovan and A. Lev-Tov. Differential Effects of Opioids on Sacrocaudal Afferent Pathways and Central Pattern Generators in the Neonatal Rat Spinal Cord.J Neurophysiol 97: 2875-2886, 2007. First published February 7, 2007; doi:10.1152/jn.01313.2006 0022-3077/07.
...MOR-1 (either from Neuromics or Chemicon, raised in rabbit, in a working dilution 1:500)...








