Tested, Characterized and Research Ready

Contact Information

Neuromics
5325 West 74th St., Suite 8
Edina, MN 55439

Toll free: 866-350-1500
Int'l phone: 952-374-6161
Fax: 612-677-3976

Featured Reagent

 MOR-1C Antibody

Related Reagents:  

Opioid Receptor Antibodies

Pain and Inflammation Research Antibodies

Neurotransmission-Neurotransmission Research Antibody Categories

Primary Neurons and Astrocytes-Primary human, rat and mouse neurons and astrocytes

 C. E. Groer, K. Tidgewell, R. A. Moyer, W. W. Harding, R. B. Rothman, T. E. Prisinzano, and L. M. Bohn. An Opioid Agonist that Does Not Induce mu-Opioid Receptor---Arrestin Interactions or Receptor Internalization. 0026-895X/07/7102-549–557. MOLECULAR PHARMACOLOGY. 

...Cells were resuspended in Triton X-100 extraction buffer [10 mM Tris, pH 7.5, 120 mM NaCl, 25 mM KCl, 1 mM PMSF, 1 protease inhibitor pellet/10 ml (Roche), 1 mg/ml iodoacetamide, and 0.5% Triton X-100] and solubilized on ice for 2 h. Lysates were cleared by 12,000 rpm spin and then added to avidin-conjugated agarose beads (Pierce) for immunoprecipitation overnight at 4°C with rotation. Immunoprecipitates were centrifuged at 12,000 rpm and washed three times with Triton X-100 extraction buffer and once with PBS plus 0.1% SDS. Immunoprecipitates were washed, and proteins were eluted and resolved as described in the coimmunoprecipitation experiments. Immunoblots were performed using the  mOR C-terminal directed antibody (Neuromics, Edina, MN), donkey anti-rabbit secondary antibody, and enhanced chemiluminescence detection...

D. Blivis, G. Z. Mentis, M. J. O'Donovan and A. Lev-Tov. Differential Effects of Opioids on Sacrocaudal Afferent Pathways and Central Pattern Generators in the Neonatal Rat Spinal Cord.J Neurophysiol 97: 2875-2886, 2007. First published February 7, 2007; doi:10.1152/jn.01313.2006 0022-3077/07.

...MOR-1 (either from Neuromics or Chemicon, raised in rabbit, in a working dilution 1:500)...