Cortex

Primary Rat Cortical Cells are live neurons isolated from micro-surgically dissected regions of day 18 embryonic Sprague/Dawley rat brain. These cells are prepared fresh each week and shipped in a nutrient rich medium that keeps the cells alive for up to 14 days under refrigeration.

Customer Publications

Images: Pak1 enhances mutant htt aggregation and toxicity in primary neurons. (A) Muhtt548-GFP/vector, muhtt548-GFP/Pak1, muhtt548-GFP/Pak1-299R were transfected into rat E18 cortical neurons. After 96 h, cell death and aggregation were evaluated as described for the cell lines. *P < 0.01; **P < 0.001. (B) Muhtt548-GFP/vector, muhtt548-GFP/Pak1 were transfected into rat E18 striatal neurons. After 96 h, cell death and aggregation were evaluated. *P < 0.01; **P < 0.001. Bar, 5 µm. (C) Examples of aggregates and nuclei in muhtt548-GFP/vector- or muhtt548-GFP/Pak1-transfected rat E18 striatal neurons. Human Molecular Genetics 2008 17(6):895-905; doi:10.1093/hmg/ddm362.

Companion Products:

NbActiv4^TM-neuron culture medium is an improved version of Neurobasaltm / B27tm.  NbActiv4 grows neurons with twice as many synapses and up to 8x higher spike rates than Neurobasal/B27.

Poly-D-Lysine Coated Coverslips-2 Glass coverslips, (1.76cm2) coated with poly-D-lysine, sterile, in PBS, shelf-life 1 week, per pair.

Please note we also provide Adult Neurons in Qtys of 4 (or more) for $995.  To order call brett @ 866-350-1500 or e-mail: brett@neuromics.com.