Apoptosis KitsFLICA-in vitro and FLIVO-in vitro |
Featured Kits
FLICA™ Polycaspase Assay Kit, green
FLIVO™ Polycaspase Live!, green
Related Reagents:
FLIVO™ Polycaspase Live!, in vivo Apoptosis Kits-New -Designed for cancer and neurodegenerative disease applications.
FLICA™ in vitro Caspase Kits -Fast!-Use Caspase kits to quantitate apoptosis via active caspases in whole, living cells. These kits do not use ELISA or any antibodies for detection.
FLISP™ Serine Protease Detection Kits -Measure chymotrypsin-like protease activation in whole living cells.
MitoPT™ Kits -Quantitate mitochondrial functionality and apoptosis
Images: Fluorescent detection of apoptosis and necrosis and related flow cytometry quantification. HL-60 cells were treated with an apoptosis-inducing agent,then dually labeled with Polycaspase Assay Kit, green and Propidium Iodide (included in the kit). Treatment with this agent induced early apoptosis in some of the cells. Courtesy of Dr.Zbigniew Darzynkiewicz.
Images: Using FLIVO to measure the response to chemotherapy.By using FAM-FLIVOTM to quantify caspase activity in vivo, it was shown that just 3 hours of treatment with arsenic trioxide (ATO) doubled the level of apoptosis in FSaII fibrosarcoma tumors in mice: 39% of tumor cells from treated animals were apoptotic vs. 18% of tumor cells from control animals. FSaII murine fibrosarcoma tumors were transplanted and grown inside a window chamber on the backs of CH3 mice. Mice were injected with saline or ATO. 3 hours post-treatment, the mice were injected with FAM-FLIVO to identify caspase-positive tumor cells; pictures were taken 30 minutes later. To quantify the level of caspase activity and apoptosis, the tumors were excised and trypsinized for analysis on a flow cytometer. By using FAM-FLIVO to quantify caspase activity in vivo, it was shown that the level of apoptosis doubled in the FSaII tumors after just 3 hours of chemotherapy.
Images: FLICA was used to assess cell death in primay rat hippocampal neurons.Cells were plated on 25-mm poly-l-lysine-coated coverslips at 300,000 cells per coverslip. Cells were used at 4 or 8 days in vitro. Composite imagae (A) 3 out of 4 cells are apoptotic (green). No cells were necrotic as both of the PI-positive cells were FLICA-positive; they had compromised membranes and were probably in the late stages of apoptosis rather than necrosis. (B) 3 Caspase-positive cells fluoresce green.